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Enzynomics co Ltd ptop blunt v2 vector
Ptop Blunt V2 Vector, supplied by Enzynomics co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptop+blunt+v2+vector/ptop+blunt+v2+vector/pm39497114-59-14-18
Average 90 stars, based on 1 article reviews
ptop blunt v2 vector - by Bioz Stars, 2026-10
90/100 stars

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Purification:

Article Title: Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone
Article Snippet: The PCR products were separated on 1.2% agarose gel and purified using a gel extraction kit (Promega, Madison, WI, USA). .. Purified DNA was ligated to a pTOP Blunt V2 vector (Enzynomics, Daejeon, Republic of Korea) and transformed into Escherichia coli DHα competent cells (Enzynomics, Daejeon, Republic of Korea). ..

Article Title: Hdh-Tektin-4 Regulates Motility of Fresh and Cryopreserved Sperm in Pacific Abalone, Haliotis discus hannai
Article Snippet: The obtained PCR products were subjected to 1.2% agarose gel electrophoresis and purified using a Wizard® SV Gel and PCR Clean-Up System kit (Promega). .. The purified DNA was ligated into pTOP Blunt V2 vector (Enzynomics, Korea) and transformed into Escherichia coli DH5α competent cells (Enzynomics, Korea). .. The plasmid DNA of positive clones was then purified using a Hybrid-QTM Plasmid Rapidprep mini kit (GeneAll, Seoul, Korea) and sequenced at Macrogen (Seoul, Korea).

Article Title: Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone.
Article Snippet: The PCR products were separated on 1.2% agarose gel and purified using a gel extraction kit (Promega, Madison, WI, USA). .. Purified DNA was ligated to a pTOP Blunt V2 vector (Enzynomics, Daejeon, Republic of Korea) and transformed into Escherichia coli DHα competent cells (Enzynomics, Daejeon, Republic of Korea). ..

Plasmid Preparation:

Article Title: Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone
Article Snippet: The PCR products were separated on 1.2% agarose gel and purified using a gel extraction kit (Promega, Madison, WI, USA). .. Purified DNA was ligated to a pTOP Blunt V2 vector (Enzynomics, Daejeon, Republic of Korea) and transformed into Escherichia coli DHα competent cells (Enzynomics, Daejeon, Republic of Korea). ..

Article Title: Hdh-Tektin-4 Regulates Motility of Fresh and Cryopreserved Sperm in Pacific Abalone, Haliotis discus hannai
Article Snippet: The obtained PCR products were subjected to 1.2% agarose gel electrophoresis and purified using a Wizard® SV Gel and PCR Clean-Up System kit (Promega). .. The purified DNA was ligated into pTOP Blunt V2 vector (Enzynomics, Korea) and transformed into Escherichia coli DH5α competent cells (Enzynomics, Korea). .. The plasmid DNA of positive clones was then purified using a Hybrid-QTM Plasmid Rapidprep mini kit (GeneAll, Seoul, Korea) and sequenced at Macrogen (Seoul, Korea).

Article Title: Improvement of succinate production from methane by combining rational engineering and laboratory evolution in Methylomonas sp. DH-1
Article Snippet: .. Amplified DNA fragments were ligated using Gibson assembly (NEB, USA) and inserted into the pTOP Blunt V2 vector (Enzynomics, Korea) to generate pTOP- ssadh . ..

Article Title: Molecular diagnosis of patients with hepatitis A virus infection using amplicon-based nanopore sequencing.
Article Snippet: .. Quantitation of HAV genome copy numbers Recombinant plasmid DNA of the HAV VP3 gene was isolated using the pTOP Blunt V2 vector (Enzynomics, Seoul, ROK). ..

Article Title: Improvement of succinate production from methane by combining rational engineering and laboratory evolution in Methylomonas sp. DH-1.
Article Snippet: .. Amplified DNA fragments were ligated using Gibson assembly (NEB, USA) and inserted into the pTOP Blunt V2 vector (Enzynomics, Korea) to generate pTOP-ssadh. ..

Article Title: Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone.
Article Snippet: The PCR products were separated on 1.2% agarose gel and purified using a gel extraction kit (Promega, Madison, WI, USA). .. Purified DNA was ligated to a pTOP Blunt V2 vector (Enzynomics, Daejeon, Republic of Korea) and transformed into Escherichia coli DHα competent cells (Enzynomics, Daejeon, Republic of Korea). ..

Article Title: Structural and functional characterization of Hdh-HSBP1 and its involvement in heat stress and early development in Pacific abalone, Haliotis discus hannai.
Article Snippet: Heat shock factor binding protein 1 (HSBP1) is known to regulate the activity of heat shock factor 1 (HSF1) and the early development of organisms.. To understand the involvement of HSBP1 in the heat shock response and embryonic and larval development of Pacific abalone (Haliotis discus hannai), the Hdh-HSBP1 gene was sequenced from the digestive gland (DG) tissue.. The full-length sequence of Hdh-HSBP1 encompassed 738 nucleotides, encoding an 8.42 kDa protein consisting of 75 deduced amino acids.

Transformation Assay:

Article Title: Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone
Article Snippet: The PCR products were separated on 1.2% agarose gel and purified using a gel extraction kit (Promega, Madison, WI, USA). .. Purified DNA was ligated to a pTOP Blunt V2 vector (Enzynomics, Daejeon, Republic of Korea) and transformed into Escherichia coli DHα competent cells (Enzynomics, Daejeon, Republic of Korea). ..

Article Title: Hdh-Tektin-4 Regulates Motility of Fresh and Cryopreserved Sperm in Pacific Abalone, Haliotis discus hannai
Article Snippet: The obtained PCR products were subjected to 1.2% agarose gel electrophoresis and purified using a Wizard® SV Gel and PCR Clean-Up System kit (Promega). .. The purified DNA was ligated into pTOP Blunt V2 vector (Enzynomics, Korea) and transformed into Escherichia coli DH5α competent cells (Enzynomics, Korea). .. The plasmid DNA of positive clones was then purified using a Hybrid-QTM Plasmid Rapidprep mini kit (GeneAll, Seoul, Korea) and sequenced at Macrogen (Seoul, Korea).

Article Title: Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone.
Article Snippet: The PCR products were separated on 1.2% agarose gel and purified using a gel extraction kit (Promega, Madison, WI, USA). .. Purified DNA was ligated to a pTOP Blunt V2 vector (Enzynomics, Daejeon, Republic of Korea) and transformed into Escherichia coli DHα competent cells (Enzynomics, Daejeon, Republic of Korea). ..

Article Title: Structural and functional characterization of Hdh-HSBP1 and its involvement in heat stress and early development in Pacific abalone, Haliotis discus hannai.
Article Snippet: Heat shock factor binding protein 1 (HSBP1) is known to regulate the activity of heat shock factor 1 (HSF1) and the early development of organisms.. To understand the involvement of HSBP1 in the heat shock response and embryonic and larval development of Pacific abalone (Haliotis discus hannai), the Hdh-HSBP1 gene was sequenced from the digestive gland (DG) tissue.. The full-length sequence of Hdh-HSBP1 encompassed 738 nucleotides, encoding an 8.42 kDa protein consisting of 75 deduced amino acids.

Amplification:

Article Title: Improvement of succinate production from methane by combining rational engineering and laboratory evolution in Methylomonas sp. DH-1
Article Snippet: .. Amplified DNA fragments were ligated using Gibson assembly (NEB, USA) and inserted into the pTOP Blunt V2 vector (Enzynomics, Korea) to generate pTOP- ssadh . ..

Article Title: Improvement of succinate production from methane by combining rational engineering and laboratory evolution in Methylomonas sp. DH-1.
Article Snippet: .. Amplified DNA fragments were ligated using Gibson assembly (NEB, USA) and inserted into the pTOP Blunt V2 vector (Enzynomics, Korea) to generate pTOP-ssadh. ..

Article Title: An Apoplastic Effector Pat-1 Cm of the Gram-Positive Bacterium Clavibacter michiganensis Acts as Both a Pathogenicity Factor and an Immunity Elicitor in Plants
Article Snippet: .. The pat-1 Cm gene and its variants, including an SP-deleted mutant and six alanine-substituted mutants with 0.4-kb native promoter region and a FLAG tag on their C-termini were amplified from C. michiganensis strain LMG7333 by PCR and cloned into the pTOP blunt V2 vector (Enzynomics, Daejeon, Korea), named pTOP- pat-1 Cm . ..

Quantitation Assay:

Article Title: Molecular diagnosis of patients with hepatitis A virus infection using amplicon-based nanopore sequencing.
Article Snippet: .. Quantitation of HAV genome copy numbers Recombinant plasmid DNA of the HAV VP3 gene was isolated using the pTOP Blunt V2 vector (Enzynomics, Seoul, ROK). ..

Recombinant:

Article Title: Molecular diagnosis of patients with hepatitis A virus infection using amplicon-based nanopore sequencing.
Article Snippet: .. Quantitation of HAV genome copy numbers Recombinant plasmid DNA of the HAV VP3 gene was isolated using the pTOP Blunt V2 vector (Enzynomics, Seoul, ROK). ..

Isolation:

Article Title: Molecular diagnosis of patients with hepatitis A virus infection using amplicon-based nanopore sequencing.
Article Snippet: .. Quantitation of HAV genome copy numbers Recombinant plasmid DNA of the HAV VP3 gene was isolated using the pTOP Blunt V2 vector (Enzynomics, Seoul, ROK). ..

Polymerase Chain Reaction:

Article Title: Structural and functional characterization of Hdh-HSBP1 and its involvement in heat stress and early development in Pacific abalone, Haliotis discus hannai.
Article Snippet: Heat shock factor binding protein 1 (HSBP1) is known to regulate the activity of heat shock factor 1 (HSF1) and the early development of organisms.. To understand the involvement of HSBP1 in the heat shock response and embryonic and larval development of Pacific abalone (Haliotis discus hannai), the Hdh-HSBP1 gene was sequenced from the digestive gland (DG) tissue.. The full-length sequence of Hdh-HSBP1 encompassed 738 nucleotides, encoding an 8.42 kDa protein consisting of 75 deduced amino acids.

Article Title: An Apoplastic Effector Pat-1 Cm of the Gram-Positive Bacterium Clavibacter michiganensis Acts as Both a Pathogenicity Factor and an Immunity Elicitor in Plants
Article Snippet: .. The pat-1 Cm gene and its variants, including an SP-deleted mutant and six alanine-substituted mutants with 0.4-kb native promoter region and a FLAG tag on their C-termini were amplified from C. michiganensis strain LMG7333 by PCR and cloned into the pTOP blunt V2 vector (Enzynomics, Daejeon, Korea), named pTOP- pat-1 Cm . ..

Mutagenesis:

Article Title: An Apoplastic Effector Pat-1 Cm of the Gram-Positive Bacterium Clavibacter michiganensis Acts as Both a Pathogenicity Factor and an Immunity Elicitor in Plants
Article Snippet: .. The pat-1 Cm gene and its variants, including an SP-deleted mutant and six alanine-substituted mutants with 0.4-kb native promoter region and a FLAG tag on their C-termini were amplified from C. michiganensis strain LMG7333 by PCR and cloned into the pTOP blunt V2 vector (Enzynomics, Daejeon, Korea), named pTOP- pat-1 Cm . ..

FLAG-tag:

Article Title: An Apoplastic Effector Pat-1 Cm of the Gram-Positive Bacterium Clavibacter michiganensis Acts as Both a Pathogenicity Factor and an Immunity Elicitor in Plants
Article Snippet: .. The pat-1 Cm gene and its variants, including an SP-deleted mutant and six alanine-substituted mutants with 0.4-kb native promoter region and a FLAG tag on their C-termini were amplified from C. michiganensis strain LMG7333 by PCR and cloned into the pTOP blunt V2 vector (Enzynomics, Daejeon, Korea), named pTOP- pat-1 Cm . ..

Clone Assay:

Article Title: An Apoplastic Effector Pat-1 Cm of the Gram-Positive Bacterium Clavibacter michiganensis Acts as Both a Pathogenicity Factor and an Immunity Elicitor in Plants
Article Snippet: .. The pat-1 Cm gene and its variants, including an SP-deleted mutant and six alanine-substituted mutants with 0.4-kb native promoter region and a FLAG tag on their C-termini were amplified from C. michiganensis strain LMG7333 by PCR and cloned into the pTOP blunt V2 vector (Enzynomics, Daejeon, Korea), named pTOP- pat-1 Cm . ..



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